Molecular and structural enzymology

Research projects developed in Enzymology and Glycochemistry group are at the frontier of chemistry and biology. To understand the molecular as well as the structural mechanism of enzymes, the group uses macromolecular crystallography and molecular modeling.

Some examples of structural projects developed by the group :

Structure of a complex between Concanavalin A and a synthetic analogue of polymannoside (pdb 4PF5) – Modeling of the binding and the bridging effect of the ligand between 2 Concanavalin A molecules.

 

α-L fucosidase (pdb 4OUE) from Bacteroides thetaiotaomicron complexed with IPTG.

 

For more information:

Synthesis of High-Mannose Oligosaccharide Analogues through Click Chemistry: True Functional Mimics of Their Natural Counterparts Against Lectins?

M.F. Heude, A. Méndez-Ardoy, V. Cendret, P. Lafite, R. Daniellou, C. Ortiz-Mellet, J. Garcia Fernandez, V. Moreau, F. Djedaïni-Pilard.

Chem. Eur. J., 2015

Unraveling the substrate recognition mechanism and specificity of the unusual glycosyl hydrolase family GH29 BT2192 from Bacteroides thtaiotaomicron.

L. Guillotin, P. Lafite, R. Daniellou

Biochemistry. 2014